Cloning Vector Collection - Detail

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Name pCas
Replicon pSC101ts
Size 12.542
Purpose Constitutive expression of cas9 and inducible expression of lambda RED and sgR
Selectable Markers kan
Other Genes repA101(Ts), kan, Pcas-cas9, ParaB-Red, lacIq Ptrc-sgRNA-pMB1
Promoter
Cloning Sites
Reference Jiang Y, Chen B, Duan C, Sun B, Yang J, Yang S.
Multigene editing in the Escherichia coli genome via the CRISPR-Cas9 system.
Appl Environ Microbiol  (2015)  81(7)   2506-14  
[PubMed ID = 25636838]  [RRC reference] 
Source S. Yang
Map
Sequence https://www.addgene.org/62225/
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