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遺伝子変異株 - 詳細

ME No. ME5305
菌株名 AG1
性別 F-
組み込みプラスミド
自律複製プラスミド
プロファージ
遺伝子型 recA1 endA1 gyrA96 thi-1 hsdR17(rk- mk+) supE44 relA1
親株(供与菌)
親株(受容菌)
変異導入方法
最終選別マーカー
選別マーカー
入手源
文献 Tenorio E, Saeki T, Fujita K, Kitakawa M, Baba T, Mori H, Isono K.
Systematic characterization of Escherichia coli genes/ORFs affecting biofilm formation.
FEMS Microbiol. Lett.  (2003)  225(1)   107-14  
[PubMed ID = 12900028]  [RRC文献情報
Wagner A, Zarecki R, Reshef L, Gochev C, Sorek R, Gophna U, Ruppin E.
Computational evaluation of cellular metabolic costs successfully predicts genes whose expression is deleterious.
Proc. Natl. Acad. Sci. U.S.A.  (2013)  110(47)   19166-71  
[PubMed ID = 24198337]  [RRC文献情報
Qimron U, Marintcheva B, Tabor S, Richardson CC.
Genomewide screens for Escherichia coli genes affecting growth of T7 bacteriophage.
Proc. Natl. Acad. Sci. U.S.A.  (2006)  103(50)   19039-44  
[PubMed ID = 17135349]  [RRC文献情報
Srividhya KV, Krishnaswamy S.
Subclassification and targeted characterization of prophage-encoded two-component cell lysis cassette.
J. Biosci.  (2007)  32(5)   979-90  
[PubMed ID = 17914239]  [RRC文献情報
Harrison JJ, Tremaroli V, Stan MA, Chan CS, Vacchi-Suzzi C, Heyne BJ, Parsek MR, Ceri H, Turner RJ.
Chromosomal antioxidant genes have metal ion-specific roles as determinants of bacterial metal tolerance.
Environ. Microbiol.  (2009)  11(10)   2491-509  
[PubMed ID = 19555372]  [RRC文献情報
Harrison JJ, Wade WD, Akierman S, Vacchi-Suzzi C, Stremick CA, Turner RJ, Ceri H.
The chromosomal toxin gene yafQ is a determinant of multidrug tolerance for Escherichia coli growing in a biofilm.
Antimicrob. Agents Chemother.  (2009)  53(6)   2253-8  
[PubMed ID = 19307375]  [RRC文献情報
Ferreira E, Giménez R, Aguilera L, Guzmán K, Aguilar J, Badia J, Baldomà L.
Protein interaction studies point to new functions for Escherichia coli glyceraldehyde-3-phosphate dehydrogenase.
Res. Microbiol.  (2013)  164(2)   145-54  
[PubMed ID = 23195894]  [RRC文献情報
Sugihara S, Orikasa Y, Okuyama H.
The Escherichia coli highly expressed entD gene complements the pfaE deficiency in a pfa gene clone responsible for the biosynthesis of long-chain n-3 polyunsaturated fatty acids.
FEMS Microbiol. Lett.  (2010)  307(2)   207-11  
[PubMed ID = 20636978]  [RRC文献情報
Ogwang S, Nguyen HT, Sherman M, Bajaksouzian S, Jacobs MR, Boom WH, Zhang GF, Nguyen L.
Bacterial conversion of folinic acid is required for antifolate resistance.
J. Biol. Chem.  (2011)  286(17)   15377-90  
[PubMed ID = 21372133]  [RRC文献情報
培地条件 Add 1 µg/ml of thiamine and 40 µg/ml of histidine into the medium, when you use minimal medium.
その他備考 AG1 has uncharacterized mutations including mutation that improves transformation efficiency and mutation that causes auxotrophy for histidine.
sequence (FASTQ format)
http://trace.ddbj.nig.ac.jp/DRASearch/run?acc=DRR058069
分譲依頼
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