Gene Mutants - Detail

ME No. ME5305
Strain Name AG1
Sex F-
Plasmid Integrated
Plasmid Autonomous
Genotype recA1 endA1 gyrA96 thi-1 hsdR17(rk- mk+) supE44 relA1
Parent Donor
Parent Recipient
Latest Marker
Reference Nosho K, Fukushima H, Asai T, Nishio M, Takamaru R, Kobayashi-Kirschvink KJ, Ogawa T, Hidaka M, Masaki H.
cAMP-CRP acts as a key regulator for the viable but non-culturable state in Escherichia coli.
Microbiology (Reading)  (2018)  164(3)   410-419  
[PubMed ID = 29458560]  [RRC reference
Tenorio E, Saeki T, Fujita K, Kitakawa M, Baba T, Mori H, Isono K.
Systematic characterization of Escherichia coli genes/ORFs affecting biofilm formation.
FEMS Microbiol Lett  (2003)  225(1)   107-14  
[PubMed ID = 12900028]  [RRC reference
Ogwang S, Nguyen HT, Sherman M, Bajaksouzian S, Jacobs MR, Boom WH, Zhang GF, Nguyen L.
Bacterial conversion of folinic acid is required for antifolate resistance.
J Biol Chem  (2011)  286(17)   15377-90  
[PubMed ID = 21372133]  [RRC reference
Sugihara S, Orikasa Y, Okuyama H.
The Escherichia coli highly expressed entD gene complements the pfaE deficiency in a pfa gene clone responsible for the biosynthesis of long-chain n-3 polyunsaturated fatty acids.
FEMS Microbiol Lett  (2010)  307(2)   207-11  
[PubMed ID = 20636978]  [RRC reference
Wagner A, Zarecki R, Reshef L, Gochev C, Sorek R, Gophna U, Ruppin E.
Computational evaluation of cellular metabolic costs successfully predicts genes whose expression is deleterious.
Proc Natl Acad Sci U S A  (2013)  110(47)   19166-71  
[PubMed ID = 24198337]  [RRC reference
Ferreira E, Giménez R, Aguilera L, Guzmán K, Aguilar J, Badia J, Baldomà L.
Protein interaction studies point to new functions for Escherichia coli glyceraldehyde-3-phosphate dehydrogenase.
Res Microbiol  (2013)  164(2)   145-54  
[PubMed ID = 23195894]  [RRC reference
Harrison JJ, Tremaroli V, Stan MA, Chan CS, Vacchi-Suzzi C, Heyne BJ, Parsek MR, Ceri H, Turner RJ.
Chromosomal antioxidant genes have metal ion-specific roles as determinants of bacterial metal tolerance.
Environ Microbiol  (2009)  11(10)   2491-509  
[PubMed ID = 19555372]  [RRC reference
Jeucken A, Helms JB, Brouwers JF.
Cardiolipin synthases of Escherichia coli have phospholipid class specific phospholipase D activity dependent on endogenous and foreign phospholipids.
Biochim Biophys Acta Mol Cell Biol Lipids  (2018)  1863(10)   1345-1353  
[PubMed ID = 29933046]  [RRC reference
Harrison JJ, Wade WD, Akierman S, Vacchi-Suzzi C, Stremick CA, Turner RJ, Ceri H.
The chromosomal toxin gene yafQ is a determinant of multidrug tolerance for Escherichia coli growing in a biofilm.
Antimicrob Agents Chemother  (2009)  53(6)   2253-8  
[PubMed ID = 19307375]  [RRC reference
Qimron U, Marintcheva B, Tabor S, Richardson CC.
Genomewide screens for Escherichia coli genes affecting growth of T7 bacteriophage.
Proc Natl Acad Sci U S A  (2006)  103(50)   19039-44  
[PubMed ID = 17135349]  [RRC reference
Srividhya KV, Krishnaswamy S.
Subclassification and targeted characterization of prophage-encoded two-component cell lysis cassette.
J Biosci  (2007)  32(5)   979-90  
[PubMed ID = 17914239]  [RRC reference
Culture Condition Add 1 µg/ml of thiamine and 40 µg/ml of histidine into the medium, when you use minimal medium.
Other Remarks AG1 has uncharacterized mutations including mutation that improves transformation efficiency and mutation that causes auxotrophy for histidine.
sequence (FASTQ format)