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Summary
| Resource Type | Hit Count |
|---|---|
| Gene Mutants | 1hits |
| Culture Condition | 1hits |
| ASKA Clone(-) | 2hits |
| ASKA Clone(+) | 2hits |
| Gateway Entry Clone library | 1hits |
| Mobile Plasmid Clone | 2hits |
| Cosmid Collection | 0hits |
| pLC Plasmid Collection | 1hits |
| Keio Collection | 2hits |
| Large-scale chromosomal deletion mutant (Tokyo Metropolitan University Group) | 1hits |
| NIG Wide Deletion Strain Collection (NEDO project) | 0hits |
| Transposon insertion disruptant | 0hits |
| Transposon insertion disruptant (cis partial doublet strain) | 2hits |
| Phage | 0hits |
| Cloning Vector Collection | 0hits |
| Cloning Vector CollectionHost Cell | 0hits |
| Antibody | 0hits |
Gene Mutants - List
Search Condition : (Keyword = hflD asuE b1132 ECK1118 hslZ JW5165 trmU ycfC)
1hits
First Previous 1-1 Next Last All| ME No. | Strain Name | Sex | Genotype | Other Remarks | Gene Mutants Detail Info | Request |
|---|---|---|---|---|---|---|
| IH150 | W3110 ts 14-21 | F- | ts |
ts phenotype is complemented with infection of Kohara phage clone 239.240.
asuE,ycfB |
 |
Culture Condition - List
Search Condition : (Keyword = hflD asuE b1132 ECK1118 hslZ JW5165 trmU ycfC)
1hits
First Previous 1-1 Next Last All| Gene Symbol | Mnemonic | Synonyms |
|---|---|---|
| asuE | asparagine utilization |
ASKA Clone(-) - List
Search Condition : (Keyword = hflD asuE b1132 ECK1118 hslZ JW5165 trmU ycfC)
2hits
First Previous 1-2 Next Last All
Resource No (JW ID)
 |
Gene Name
|
Primer Sequence for N terminal | Primer Sequence for C terminal | Comment | Request |
|---|---|---|---|---|---|
| JW5165-AM | hflD | GCCGCAAAGAATTACTATGACAT | CCtAACTCCGGGGTTAAATGAGC | Clone OK | |
| JW1119-AM | trmU | GCCTCTGAAACCGCAAAAAAAGT | CCGACCGGCAGCGGCAGACGCTG | Clone OK |
ASKA Clone(+) - List
Search Condition : (Keyword = hflD asuE b1132 ECK1118 hslZ JW5165 trmU ycfC)
2hits
First Previous 1-2 Next Last All|
Resource No (JW ID)
|
Gene Name
|
Primer Sequence for N terminal | Primer Sequence for C terminal | Comment | Request |
|---|---|---|---|---|---|
| JW5165-AP | hflD | GCCGCAAAGAATTACTATGACAT | CCtAACTCCGGGGTTAAATGAGC | Clone OK | |
| JW1119-AP | trmU | GCCTCTGAAACCGCAAAAAAAGT | CCGACCGGCAGCGGCAGACGCTG | Clone OK |
Gateway Entry Clone library - List
Search Condition : (Keyword = hflD asuE b1132 ECK1118 hslZ JW5165 trmU ycfC)
1hits
First Previous 1-1 Next Last All|
Resource No (JW ID)
|
Gene Name
|
Clone verification class | Cloning Method | Request |
|---|---|---|---|---|
| JW1119-GE | trmU | A | ASKA to pDONR/zeo |
Mobile Plasmid Collection - List
Search Condition : (Keyword = hflD asuE b1132 ECK1118 hslZ JW5165 trmU ycfC)
2hits
First Previous 1-2 Next Last All| Accession No | ORF No.(Japan) | ORF No.(USA) | Gene Name | Length | Source | Plasmid | Request |
|---|---|---|---|---|---|---|---|
| 1085 | 240#3 | b1132 | ycfC | 648 | Nara | pNT3 | |
| 1086 | 240#4 | b1133 | trmU | 1107 | USA | pNTR-SD |
pLC Plasmid - List
Search Condition : (Keyword = hflD asuE b1132 ECK1118 hslZ JW5165 trmU ycfC)
1hits
First Previous 1-1 Next Last All| pLC Plasmid | Map Position(min)a | Kohara clones by hybridizedb and genesc | Request | |
|---|---|---|---|---|
| 3-2 | 3-2 | * | purB, asuE, icd(25 min) | |
| 25 | E4C2(238), 7F9(239) |
Keio Collection - List
Search Condition : (Keyword = hflD asuE b1132 ECK1118 hslZ JW5165 trmU ycfC)
2hits
First Previous 1-2 Next Last All
Large-scale chromosomal deletion mutant (Tokyo Metropolitan University Group) - List
Search Condition : (Keyword = hflD asuE b1132 ECK1118 hslZ JW5165 trmU ycfC)
1hits
First Previous 1-1 Next Last All
