Gene Mutants - Detail

ME No. ME8452
Strain Name JW1071
Sex Hfr
Plasmid Integrated F HfrPO1 of HfrH(valS <- uxuAB)
Plasmid Autonomous
Prophage
Genotype zbf-507::Tn10 trp-49 lacZ125 relA1 spoT1 (lambda)-
Parent Donor
Parent Recipient
Methods
Latest Marker
Selection
Source
Gene Mutants Detail Info
Reference Winkelman JW, Clark DP.
Proton suicide: general method for direct selection of sugar transport- and fermentation-defective mutants.
J Bacteriol  (1984)  160(2)   687-90  
[PubMed ID = 6094484]  [RRC reference] 
Pahel G, Bloom FR, Tyler B.
Deletion mapping of the polA-metB region of the Escherichia coli chromosome.
J Bacteriol  (1979)  138(2)   653-6  
[PubMed ID = 35528]  [RRC reference] 
Shuman HA.
Active transport of maltose in Escherichia coli K12. Role of the periplasmic maltose-binding protein and evidence for a substrate recognition site in the cytoplasmic membrane.
J Biol Chem  (1982)  257(10)   5455-61  
[PubMed ID = 7040366]  [RRC reference] 
Culture Condition
Other Remarks Co-td supE/zbf-507::Tn10 : 47% pgm /zbf-507::Tn10 : 93% trp-49 = Brenner's trp-8(Am) lacZ125 = Jacob's lacY14(Am)
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