Gene Mutants - Detail

ME No. ME8298
Strain Name TL212
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype zce-726::Tn10 araD139 DE(argF-lac)U169 rpsL150 deoC1 relA1 thiA ptsF25 flbB5301 malTC-1 DE(malE)444
Parent Donor
Parent Recipient
Methods
Latest Marker
Selection
Source
Reference Shuman HA.
Active transport of maltose in Escherichia coli K12. Role of the periplasmic maltose-binding protein and evidence for a substrate recognition site in the cytoplasmic membrane.
J Biol Chem  (1982)  257(10)   5455-61  
[PubMed ID = 7040366]  [RRC reference
Winkelman JW, Clark DP.
Proton suicide: general method for direct selection of sugar transport- and fermentation-defective mutants.
J Bacteriol  (1984)  160(2)   687-90  
[PubMed ID = 6094484]  [RRC reference
Pahel G, Bloom FR, Tyler B.
Deletion mapping of the polA-metB region of the Escherichia coli chromosome.
J Bacteriol  (1979)  138(2)   653-6  
[PubMed ID = 35528]  [RRC reference
Culture Condition
Other Remarks Co-td ptsG/zce-726::Tn10 : 80%
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