Gene Mutants - Detail

ME No. ME8294
Strain Name ET1214
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype zig-219::Tn10 thi-1 lacY1 gal-6 malA1 xyl-7 mtl-2 rpsL9 tonA2 tsx-68 (lambda)- supE44 lambda_R
Parent Donor
Parent Recipient
Methods
Latest Marker
Selection
Source
Gene Mutants Detail Info
Reference Shuman HA.
Active transport of maltose in Escherichia coli K12. Role of the periplasmic maltose-binding protein and evidence for a substrate recognition site in the cytoplasmic membrane.
J Biol Chem  (1982)  257(10)   5455-61  
[PubMed ID = 7040366]  [RRC reference] 
Winkelman JW, Clark DP.
Proton suicide: general method for direct selection of sugar transport- and fermentation-defective mutants.
J Bacteriol  (1984)  160(2)   687-90  
[PubMed ID = 6094484]  [RRC reference] 
Pahel G, Bloom FR, Tyler B.
Deletion mapping of the polA-metB region of the Escherichia coli chromosome.
J Bacteriol  (1979)  138(2)   653-6  
[PubMed ID = 35528]  [RRC reference] 
Culture Condition
Other Remarks zig-219::Tn10 = zig-2::Tn10 Co-td tpi/zig-219::Tn10 : 44% glnA/zig-219::Tn10 : 85% rha/zig-219::Tn10 : 20% pfkA
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