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Gene Mutants - Detail

ME No. ME8290
Strain Name BW9116
Sex Hfr
Plasmid Integrated F HfrKL16
Plasmid Autonomous
Prophage
Genotype thi-1 DE(xthA-pncA)90 relA1 spoT1? zdh-201::Tn10
Parent Donor
Parent Recipient
Methods
Latest Marker
Selection
Source
Reference Pahel G, Bloom FR, Tyler B.
Deletion mapping of the polA-metB region of the Escherichia coli chromosome.
J. Bacteriol.  (1979)  138(2)   653-6  
[PubMed ID = 35528]  [RRC reference
Shuman HA.
Active transport of maltose in Escherichia coli K12. Role of the periplasmic maltose-binding protein and evidence for a substrate recognition site in the cytoplasmic membrane.
J. Biol. Chem.  (1982)  257(10)   5455-61  
[PubMed ID = 7040366]  [RRC reference
Winkelman JW, Clark DP.
Proton suicide: general method for direct selection of sugar transport- and fermentation-defective mutants.
J. Bacteriol.  (1984)  160(2)   687-90  
[PubMed ID = 6094484]  [RRC reference
Culture Condition
Other Remarks zdh-201::Tn10; near ackB, co-transduced gap pyk
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