Gene Mutants - Detail

ME No. ME8287
Strain Name 8
Sex Hfr
Plasmid Integrated F HfrC(purE -> lip)
Plasmid Autonomous
Prophage λ+
Genotype phoA8 glpD3 glpR2 relA1 tonA22 (lambda)+
Parent Donor
Parent Recipient
Methods
Latest Marker
Selection
Source Hayashi et al(1964)
Gene Mutants Detail Info
Reference Schweizer H, Boos W.
Transfer of the delta (argF-lac)U169 mutation between Escherichia coli strains by selection for a closely linked Tn10 insertion.
Mol Gen Genet  (1983)  192(1-2)   293-4  
[PubMed ID = 6316111]  [RRC reference] 
Cozzarelli NR, Koch JP, Hayashi S, Lin EC.
Growth stasis by accumulated L-alpha-glycerophosphate in Escherichia coli.
J Bacteriol  (1965)  90(5)   1325-9  
[PubMed ID = 5321485]  [RRC reference] 
Culture Condition
Other Remarks glpD strains should be maintained on media containing G3P or glycerol (LB tryptone, antibiotic medium 3 etc) only in the presence of 10mM glucose to avoid groth stasis.
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