Gene Mutants - Detail

ME No. ME8913
Strain Name CAG12122
Plasmid Integrated
Plasmid Autonomous
Genotype zea-3125::Tn10kan
Parent Donor
Parent Recipient CAG12074(ME8819)
Methods OTHER METHODS(Tn10kan conversion)
Latest Marker zea-3125::Tn10kan
Selection KmR
Source M. Singer
Reference Rath D, Mangoli SH, Mahajan SK, Jawali N.
A novel mutation spatially remote from the G-domain in IF2 affects the cold stress adaptation of Escherichia coli.
Res Microbiol  (2009)  160(8)   576-80  
[PubMed ID = 19716413]  [RRC reference
Singer M, Baker TA, Schnitzler G, Deischel SM, Goel M, Dove W, Jaacks KJ, Grossman AD, Erickson JW, Gross CA.
A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli.
Microbiol Rev  (1989)  53(1)   1-24  
[PubMed ID = 2540407]  [RRC reference
Culture Condition
Other Remarks Plaque forming activity against MG1655 was detected from culture supernatant of some strains of CAG series possibly caused by lysogenized phage.
Please be careful before using this strain.