Gene Mutants - Detail

ME No. ME10091
Strain Name eCOMB
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype ΔthyA Δ(yjjG-deoB)
Parent Donor
Parent Recipient MG1655
Methods
Latest Marker
Selection
Source
Gene Mutants Detail Info
Reference Pham TM, Tan KW, Sakumura Y, Okumura K, Maki H, Akiyama MT.
A single-molecule approach to DNA replication in Escherichia coli cells demonstrated that DNA polymerase III is a major determinant of fork speed.
Mol Microbiol  (2013)  90(3)   584-96  
[PubMed ID = 23998701]  [RRC reference] 
Culture Condition Medium supplemented with thymidine (2 μg/ml or more)
Other Remarks Recombinant status：non-recombinant
Phenotype：Requires thymidine
BrdU pulse labeling possible in 1-4 minutes (56/2 synthetic medium, 50 μg/ml BrdU)
Request Not available
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