Gene Mutants - Detail

ME No. ME9882
Strain Name SN1248
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype ΔhsdR ΔendA ΔlacZ::(T7 gene1:frt)
Parent Donor
Parent Recipient MG1655
Methods
Latest Marker
Selection
Source S. Nozaki
Reference
Culture Condition Leaky expression of T7 RNA polymerase was observed in L medium without IPTG. Addition of glucose into the medium significantly reduced the leaky expression. Transcription from the T7 promoter can be induced in L medium without glucose or L medium with IPTG.
Other Remarks iVEC express (lac), an iVEC strain for protein expression.
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