Gene Mutants - Detail

ME No. ME9785
Strain Name GN10Y
Sex
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype ΔpssA10::cam /pMS5(pssA+ derivative of pMAN035) lacY::Tn5
Parent Donor
Parent Recipient W3110
Methods The procedure for the construction of the strain is described in the paper entitled "A regulatory mechanism for the balanced syntheis of membrane phospholipid species in Escherichia coli " (Biosci. Biotech. Biochim. (1996) 60, 111-116).Curering at 43◦C for 3 hr of covering plasmid pMS5 (pssA+) in LB medium containing 50mM MgCl2, confirm Ap-sensitivity for the curing and of lacY::Tn5.
Latest Marker cam, Kan, Aps
Selection
Source
Gene Mutants Detail Info
Reference
Culture Condition LB +50 mM MgCl2
Other Remarks
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