Gene Mutants - Detail

ME No. ME8996
Strain Name AD202
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype ompT::Km DE(argF-lac)U169 araD139 relA1 rpsL150 flbB5301 deoC1 tonA21 thi ptsF25
Parent Donor AD179
Parent Recipient ME8286(=MC4100)
Methods
Latest Marker
Selection
Source K. Ito
Gene Mutants Detail Info
Reference Ryoji Miyazaki, Mengting Ai, Natsuko Tanaka, Takehiro Suzuki, Naoshi Dhomae, Tomoya Tsukazaki, Yoshinori Akiyama, Hiroyuki Mori
Inner membrane YfgM–PpiD heterodimer acts as a functional unit that associates with the SecY/E/G translocon and promotes protein translocation
Journal of Biological Chemistry  (2022)  298   102572  
[PubMed ID = 36209828]  [RRC reference] 
Akiyama Y, Ito K.
SecY protein, a membrane-embedded secretion factor of E. coli, is cleaved by the ompT protease in vitro.
Biochem Biophys Res Commun  (1990)  167(2)   711-5  
[PubMed ID = 2182019]  [RRC reference] 
Culture Condition
Other Remarks Km 10_micro_g/ml AD202=MC4100 OmpT::Km
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