Gene Mutants - Detail

ME No. ME7982
Strain Name JM109 pDsbCD1
Sex F-
Plasmid Integrated
Plasmid Autonomous Other
Parent Donor
Parent Recipient JM109
Methods TF.
Latest Marker pDsbCD1
Selection Chloramphenicol resistance
Reference Kurokawa Y, Yanagi H, Yura T.
Overexpression of protein disulfide isomerase DsbC stabilizes multiple-disulfide-bonded recombinant protein produced and transported to the periplasm in Escherichia coli.
Appl. Environ. Microbiol.  (2000)  66(9)   3960-5  
[PubMed ID = 10966415]  [RRC reference
Van Houdt R, Moons P, Aertsen A, Jansen A, Vanoirbeek K, Daykin M, Williams P, Michiels CW.
Characterization of a luxI/luxR-type quorum sensing system and N-acyl-homoserine lactone-dependent regulation of exo-enzyme and antibacterial component production in Serratia plymuthica RVH1.
Res. Microbiol.  (2007)  158(2)   150-8  
[PubMed ID = 17258895]  [RRC reference
Miyakawa T, Sawano Y, Miyazono K, Hatano K, Tanokura M.
Crystallization and preliminary X-ray analysis of ginkbilobin-2 from Ginkgo biloba seeds: a novel antifungal protein with homology to the extracellular domain of plant cysteine-rich receptor-like kinases.
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun.  (2007)  63(Pt 9)   737-9  
[PubMed ID = 17768341]  [RRC reference
Culture Condition L broth plus chloramphenicol at 37C
Other Remarks Expression plasmid for DsbCD