Gene Mutants - Detail

ME No. ME7981
Strain Name HI1006 pKV1561
Sex F-
Plasmid Integrated
Plasmid Autonomous Other
Genotype araD139 Δ(araABIOC-Leu)7697 Δ(lacIPOZY)x74 galU galK
Parent Donor
Parent Recipient HI1006
Methods TF.
Latest Marker Plasmid pKV1561
Selection Ampicillin resistance
Reference Kanemori M, Mori H, Yura T.
Effects of reduced levels of GroE chaperones on protein metabolism: enhanced synthesis of heat shock proteins during steady-state growth of Escherichia coli.
J. Bacteriol.  (1994)  176(14)   4235-42  
[PubMed ID = 7912695]  [RRC reference
Culture Condition L broth plus ampicillin at 30C
Other Remarks Expression plasmid for GroES and GroEL