Gene Mutants - Detail

ME No. ME7978
Strain Name MC4100 pKV6
Sex F-
Plasmid Integrated
Plasmid Autonomous Other
Genotype Δ(argF-lac)U169 araD139 rpsL150 thiA1 relA1 flbB5301 deoC1 ptsF25 rbsR
Parent Donor
Parent Recipient MC4100
Methods TF.
Latest Marker Plasmid pKV6 [pKV2 carrying the rpoH165(Amber) mutation]
Selection Ampicillin resistance
Reference Yura T, Tobe T, Ito K, Osawa T.
Heat shock regulatory gene (htpR) of Escherichia coli is required for growth at high temperature but is dispensable at low temperature.
Proc Natl Acad Sci U S A  (1984)  81(21)   6803-7  
[PubMed ID = 6387714]  [RRC reference
Culture Condition L broth plus ampicillin at 37C
Other Remarks