Gene Mutants - Detail

ME No. ME7976
Strain Name MC4100 pKV1
Sex F-
Plasmid Integrated
Plasmid Autonomous Other
Prophage
Genotype Δ(argF-lac)U169 araD139 rpsL150 thiA1 relA1 flbB5301 deoC1 ptsF25 rbsR
Parent Donor
Parent Recipient MC4100
Methods TF.
Latest Marker Plasmid pKV1
Selection Ampicillin resistance
Source
Reference Yura T, Tobe T, Ito K, Osawa T.
Heat shock regulatory gene (htpR) of Escherichia coli is required for growth at high temperature but is dispensable at low temperature.
Proc Natl Acad Sci U S A  (1984)  81(21)   6803-7  
[PubMed ID = 6387714]  [RRC reference
Yano R, Imai M, Yura T.
The use of operon fusions in studies of the heat-shock response: effects of altered sigma 32 on heat-shock promoter function in Escherichia coli.
Mol Gen Genet  (1987)  207(1)   24-8  
[PubMed ID = 3299002]  [RRC reference
Casadaban MJ.
Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu.
J Mol Biol  (1976)  104(3)   541-55  
[PubMed ID = 781293]  [RRC reference
Tobe T, Ito K, Yura T.
Isolation and physical mapping of temperature-sensitive mutants defective in heat-shock induction of proteins in Escherichia coli.
Mol Gen Genet  (1984)  195(1-2)   10-6  
[PubMed ID = 6092838]  [RRC reference
Culture Condition L broth plus ampicillin at 37C
Other Remarks
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