Gene Mutants - Detail

ME No. ME7975
Strain Name MC4100 (λGF364)
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage λ+ λGF364 carrying a translational (gene) fusion [rpoH(1-364)-lacZ]
Genotype Δ(argF-lac)U169 araD139 rpsL150 thiA1 relA1 flbB5301 deoC1 ptsF25 rbsR
Parent Donor
Parent Recipient MC4100
Methods OTHER METHODSLysogenization
Latest Marker Prophage λGF364 carrying a gene fusion rpoH(base 1-364)-lacZ
Reference Nagai H, Yuzawa H, Yura T.
Interplay of two cis-acting mRNA regions in translational control of sigma 32 synthesis during the heat shock response of Escherichia coli.
Proc Natl Acad Sci U S A  (1991)  88(23)   10515-9  
[PubMed ID = 1961716]  [RRC reference
Culture Condition L broth at 37C
Other Remarks λ prophage reporter for translation of rpoH (the resulting fusion protein is stable)