Gene Mutants - Detail

ME No. ME7974
Strain Name MC4100(λGF807)
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage λ+ λGF807 carrying a translational (gene) fusion rpoH-lacZ
Genotype Δ(argF-lac)U169 araD139 rpsL150 thiA1 relA1 flbB5301 deoC1 ptsF25 rbsR
Parent Donor
Parent Recipient MC4100
Methods OTHER METHODSLysogenization
Latest Marker Prophage λGF807 carrying gene fusion rpoH(base 1-807)-lacZ
Selection
Source
Reference Nagai H, Yuzawa H, Yura T.
Interplay of two cis-acting mRNA regions in translational control of sigma 32 synthesis during the heat shock response of Escherichia coli.
Proc Natl Acad Sci U S A  (1991)  88(23)   10515-9  
[PubMed ID = 1961716]  [RRC reference
Culture Condition L broth at 37C
Other Remarks λ prophage reporter for translation of rpoH (the resulting fusion protein is quite unstable)
Request
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