Gene Mutants - Detail

ME No. ME7971
Strain Name MC4100[λpF13-(PrpoDhs-lacZ)]
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage λ+ λpF13-(PrpoDhs-lacZ); Transcriptional (operon) fusion
Genotype Δ(argF-lac)U169 araD139 rpsL150 thiA1 relA1 flbB5301 deoC1 ptsF25 rbsR
Parent Donor
Parent Recipient MC4100
Methods OTHER METHODSLysogenization
Latest Marker Prophage λpF13-(PrpoDhs-lacZ)
Selection λ phage immunity
Source
Reference Casadaban MJ.
Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu.
J. Mol. Biol.  (1976)  104(3)   541-55  
[PubMed ID = 781293]  [RRC reference] 
Yura T, Tobe T, Ito K, Osawa T.
Heat shock regulatory gene (htpR) of Escherichia coli is required for growth at high temperature but is dispensable at low temperature.
Proc. Natl. Acad. Sci. U.S.A.  (1984)  81(21)   6803-7  
[PubMed ID = 6387714]  [RRC reference] 
Tobe T, Ito K, Yura T.
Isolation and physical mapping of temperature-sensitive mutants defective in heat-shock induction of proteins in Escherichia coli.
Mol. Gen. Genet.  (1984)  195(1-2)   10-6  
[PubMed ID = 6092838]  [RRC reference] 
Yano R, Imai M, Yura T.
The use of operon fusions in studies of the heat-shock response: effects of altered sigma 32 on heat-shock promoter function in Escherichia coli.
Mol. Gen. Genet.  (1987)  207(1)   24-8  
[PubMed ID = 3299002]  [RRC reference] 
Culture Condition L broth at 30C
Other Remarks λ prophage reporter for transcription from the rpoDhs heat shock promoter
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