Gene Mutants - Detail

ME No. ME7966
Strain Name KY1156
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage λ+ λCharon25-lacUV5p-groESgroEL
Genotype ΔgroE68::tet Δ(argF-lac)U169 araD139 rpsL150 thiA1 relA1 flbB5301 deoC1 ptsF25 rbsR
Parent Donor KY1880
Parent Recipient MC4100 (λCharon25-lacUV5p-groESgroEL)
Methods TD./PI
Latest Marker ΔgroE68::tet
Selection Tetracycline resistance
Reference Miyazaki T, Yoshimi T, Furutsu Y, Hongo K, Mizobata T, Kanemori M, Kawata Y.
GroEL-substrate-GroES ternary complexes are an important transient intermediate of the chaperonin cycle.
J Biol Chem  (2002)  277(52)   50621-8  
[PubMed ID = 12377767]  [RRC reference
Culture Condition L broth containing 1mM IPTG at 37C
Other Remarks Requires IPTG for maximum growth