Gene Mutants - Detail

ME No. ME7965
Strain Name KY1880
Sex F-
Plasmid Integrated
Plasmid Autonomous Other
Genotype ΔgroE68::tet Δ(argF-lac)U169 araD139 rpsL150 thiA1 relA1 flbB5301 deoC1 ptsF25 rbsR
Parent Donor
Parent Recipient MC4100 pKV1561
Methods TD./PI
Latest Marker ΔgroE68::tet
Selection Tetracycline resistance
Reference Kanemori M, Mori H, Yura T.
Effects of reduced levels of GroE chaperones on protein metabolism: enhanced synthesis of heat shock proteins during steady-state growth of Escherichia coli.
J Bacteriol  (1994)  176(14)   4235-42  
[PubMed ID = 7912695]  [RRC reference
Culture Condition L broth containing 50mM IPTG at37C
Other Remarks Requires IPTG for growth particularly at high temperature (42C)