Gene Mutants - Detail

ME No. ME7964
Strain Name NRK156
Sex F-
Plasmid Integrated
Plasmid Autonomous
Genotype dnaK756 thr34::Tn10 Δ(argF-lac)U169 araD139 rpsL150 thiA1 relA1 flbB5301 deoC1 ptsF25 rbsR
Parent Donor
Parent Recipient MC4100
Methods TD./PI
Latest Marker dnaK756 thr34::Tn10
Selection Tetracycline resistance
Source N. Kusukawa
Reference Kawasaki Y, Wada C, Yura T.
Roles of Escherichia coli heat shock proteins DnaK, DnaJ and GrpE in mini-F plasmid replication.
Mol Gen Genet  (1990)  220(2)   277-82  
[PubMed ID = 2183004]  [RRC reference
Tilly K, McKittrick N, Zylicz M, Georgopoulos C.
The dnaK protein modulates the heat-shock response of Escherichia coli.
Cell  (1983)  34(2)   641-6  
[PubMed ID = 6311435]  [RRC reference
Culture Condition L broth at 30C
Other Remarks Unable to grow at high temperature (42C)