Gene Mutants - Detail

ME No. ME7963
Strain Name NRK117
Sex F-
Plasmid Integrated
Plasmid Autonomous
Genotype groEL44 zje::Tn10 Δ(argF-lac)U169 araD139 rpsL150 thiA1 relA1 flbB5301 deoC1 ptsF25 rbsR
Parent Donor
Parent Recipient MC4100
Methods TD./PI
Latest Marker groEL44 zje::Tn10
Selection Tetracycline resistance
Reference Kusukawa N, Yura T, Ueguchi C, Akiyama Y, Ito K.
Effects of mutations in heat-shock genes groES and groEL on protein export in Escherichia coli.
EMBO J  (1989)  8(11)   3517-21  
[PubMed ID = 2573517]  [RRC reference
Culture Condition L broth at 30C
Other Remarks Unable to grow at high temperature (42C)