Gene Mutants - Detail

ME No. ME7954
Strain Name KY1612 pgroE
Sex F-
Plasmid Integrated
Plasmid Autonomous Other
Prophage λ+ λpF13-(PgroE-lacZ)
Genotype ΔrpoH30::kan zhf50::Tn10 Δ(argF-lac)U169 araD139 rpsL150 thiA1 relA1 flbB5301 deoC1 ptsF25 rbsR
Parent Donor
Parent Recipient KY1612
Methods TF.
Latest Marker Plasmid pOF12
Selection Ampicillin resistance
Reference Kusukawa N, Yura T.
Heat shock protein GroE of Escherichia coli: key protective roles against thermal stress.
Genes Dev  (1988)  2(7)   874-82  
[PubMed ID = 2905317]  [RRC reference
Culture Condition L broth at 30C
Other Remarks Unable to grow at 42C