Gene Mutants - Detail

ME No. ME7953
Strain Name W3110 DrpoH::kan
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage λ+
Genotype ΔrpoH30::kan zhf50::Tn10
Parent Donor
Parent Recipient W3110
Methods TD./PI
Latest Marker ΔrpoH30::kan zhf50::Tn10
Selection Tetracycline resistance
Source
Gene Mutants Detail Info
Reference Zhou YN, Kusukawa N, Erickson JW, Gross CA, Yura T.
Isolation and characterization of Escherichia coli mutants that lack the heat shock sigma factor sigma 32.
J Bacteriol  (1988)  170(8)   3640-9  
[PubMed ID = 2900239]  [RRC reference] 
Kusukawa N, Yura T.
Heat shock protein GroE of Escherichia coli: key protective roles against thermal stress.
Genes Dev  (1988)  2(7)   874-82  
[PubMed ID = 2905317]  [RRC reference] 
Culture Condition L broth at 20C
Other Remarks Unable to grow at or above 30C
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