Gene Mutants - Detail

ME No. ME7949
Strain Name KY1612; MC4100 DrpoH::kan
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage λ+ λpF13-(PgroE-lacZ)
Genotype ΔrpoH30::kan zhf50::Tn10 Δ(argF-lac)U169 araD139 rpsL150 thiA1 relA1 flbB5301 deoC1 ptsF25 rbsR
Parent Donor
Parent Recipient MC4100 [λpF13-(PgroE-lacZ)]
Methods TD./PI
Latest Marker ΔrpoH::kan zhf50::Tn10
Selection Tetracycline resistance
Reference Zhou YN, Kusukawa N, Erickson JW, Gross CA, Yura T.
Isolation and characterization of Escherichia coli mutants that lack the heat shock sigma factor sigma 32.
J Bacteriol  (1988)  170(8)   3640-9  
[PubMed ID = 2900239]  [RRC reference
Kusukawa N, Yura T.
Heat shock protein GroE of Escherichia coli: key protective roles against thermal stress.
Genes Dev  (1988)  2(7)   874-82  
[PubMed ID = 2905317]  [RRC reference
Culture Condition L broth at 20C
Other Remarks Unable to grow at or above 30C