Gene Mutants - Detail

ME No. ME7949
Strain Name KY1612; MC4100 DrpoH::kan
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage λ+ λpF13-(PgroE-lacZ)
Genotype ΔrpoH30::kan zhf50::Tn10 Δ(argF-lac)U169 araD139 rpsL150 thiA1 relA1 flbB5301 deoC1 ptsF25 rbsR
Parent Donor
Parent Recipient MC4100 [λpF13-(PgroE-lacZ)]
Methods TD./PI
Latest Marker ΔrpoH::kan zhf50::Tn10
Selection Tetracycline resistance
Source
Gene Mutants Detail Info
Reference Kusukawa N, Yura T.
Heat shock protein GroE of Escherichia coli: key protective roles against thermal stress.
Genes Dev  (1988)  2(7)   874-82  
[PubMed ID = 2905317]  [RRC reference] 
Zhou YN, Kusukawa N, Erickson JW, Gross CA, Yura T.
Isolation and characterization of Escherichia coli mutants that lack the heat shock sigma factor sigma 32.
J Bacteriol  (1988)  170(8)   3640-9  
[PubMed ID = 2900239]  [RRC reference] 
Culture Condition L broth at 20C
Other Remarks Unable to grow at or above 30C
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