Gene Mutants - Detail

ME No. ME7945
Strain Name MC4100 rpoH165
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage λ+
Genotype rpoH165(Amber) zhf50::Tn10 Δ(argF-lac)U169 araD139 rpsL150 thiA1 relA1 flbB5301 deoC1 ptsF25 rbsR
Parent Donor
Parent Recipient MC4100
Methods TD./PI
Latest Marker rpoH165(Amber) zhf50::Tn10
Selection Tetracycline resistance
Reference Yura T, Tobe T, Ito K, Osawa T.
Heat shock regulatory gene (htpR) of Escherichia coli is required for growth at high temperature but is dispensable at low temperature.
Proc Natl Acad Sci U S A  (1984)  81(21)   6803-7  
[PubMed ID = 6387714]  [RRC reference
Culture Condition L broth at 30C
Other Remarks Unable to grow at high temperature (42C)