Gene Mutants - Detail

ME No. ME7932
Strain Name KY1430
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype rpoH11(Ochre) zhf50::Tn10 Δ(argF-lac)U169 araD139 rpsL150 thiA1 relA1 flbB5301 deoC1 ptsF25 rbsR
Parent Donor
Parent Recipient MC4100
Methods TD./PI
Latest Marker rpoH11(Ochre) zhf50::Tn10
Selection Tetracycline resistance
Source
Reference Tobe T, Ito K, Yura T.
Isolation and physical mapping of temperature-sensitive mutants defective in heat-shock induction of proteins in Escherichia coli.
Mol. Gen. Genet.  (1984)  195(1-2)   10-6  
[PubMed ID = 6092838]  [RRC reference] 
Casadaban MJ.
Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu.
J. Mol. Biol.  (1976)  104(3)   541-55  
[PubMed ID = 781293]  [RRC reference] 
Yura T, Tobe T, Ito K, Osawa T.
Heat shock regulatory gene (htpR) of Escherichia coli is required for growth at high temperature but is dispensable at low temperature.
Proc. Natl. Acad. Sci. U.S.A.  (1984)  81(21)   6803-7  
[PubMed ID = 6387714]  [RRC reference] 
Yano R, Imai M, Yura T.
The use of operon fusions in studies of the heat-shock response: effects of altered sigma 32 on heat-shock promoter function in Escherichia coli.
Mol. Gen. Genet.  (1987)  207(1)   24-8  
[PubMed ID = 3299002]  [RRC reference] 
Culture Condition L broth at 30C
Other Remarks Unable to grow at high temperature (42C)
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