Gene Mutants - Detail

ME No. ME7806
Strain Name SH2707
Sex F-
Plasmid Integrated
Plasmid Autonomous Other
Prophage
Genotype ilv thr trp metE tyr pro(A ore B) rpsL lacI recA1 thyA(low)
Parent Donor
Parent Recipient KZ344
Methods TF.
Latest Marker pXX325
Selection Amp resistant
Source
Gene Mutants Detail Info
Reference Ogura T, Hiraga S.
Mini-F plasmid genes that couple host cell division to plasmid proliferation.
Proc Natl Acad Sci U S A  (1983)  80(15)   4784-8  
[PubMed ID = 6308648]  [RRC reference
Culture Condition LB + Amp (25 μg/ml), 37C
Other Remarks low copy plasmid, Amp should be low.
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