Notice: Suspension of Services Due to the Server maintenance work of NIG

The services on our website will be stopped during the period of time stated below due to the server maintenance work of NIG. We apologize for the inconvenience and we thank you for your understanding in this matter.

Thu. 26th Nov. 2020 18:00 - Sun. 29th Nov. 2020 12:00

Gene Mutants - Detail

ME No. ME9616
Strain Name AQ10955
Sex F+
Plasmid Integrated
Plasmid Autonomous F'
Genotype trpA9605 his-29 thyA deoB (or deoC) metB1 ilv polA25::spc zih::Tn10 dnaA5 rnhA224
Parent Donor AQ8864
Parent Recipient AQ10915
Methods F'-duction
Latest Marker F' klenow
Selection Cm-r, Lac+
Reference Kogoma T, Maldonado RR.
DNA polymerase I in constitutive stable DNA replication in Escherichia coli.
J Bacteriol  (1997)  179(7)   2109-15  
[PubMed ID = 9079893]  [RRC reference
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks Tr
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.