Gene Mutants - Detail

ME No. ME9616
Strain Name AQ10955
Sex F+
Plasmid Integrated
Plasmid Autonomous F'
Prophage
Genotype trpA9605 his-29 thyA deoB (or deoC) metB1 ilv polA25::spc zih::Tn10 dnaA5 rnhA224
Parent Donor AQ8864
Parent Recipient AQ10915
Methods F'-duction
Latest Marker F' klenow
Selection Cm-r, Lac+
Source
Reference Kogoma T, Maldonado RR.
DNA polymerase I in constitutive stable DNA replication in Escherichia coli.
J. Bacteriol.  (1997)  179(7)   2109-15  
[PubMed ID = 9079893]  [RRC reference
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks Tr
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.
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