Gene Mutants - Detail

ME No. ME9609
Strain Name AQ10907
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype trpA9605 his-29 thyA deoB (or deoC) metD88 Δlac3 ilv dnaA5 rnhA224 polA1 zig::Tn10
Parent Donor AQ1409
Parent Recipient AQ10884
Methods TD./PI
Latest Marker UVs, polA1
Selection Tc-r
Source
Reference Kogoma T, Maldonado RR.
DNA polymerase I in constitutive stable DNA replication in Escherichia coli.
J. Bacteriol.  (1997)  179(7)   2109-15  
[PubMed ID = 9079893]  [RRC reference
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.
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