Gene Mutants - Detail

ME No. ME9603
Strain Name AQ10838
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype trpA9605 his-29 proB (or proC) ilv metB1 thyA deoB (or deoC) rnhA224 ΔpolB::spc zac-3051::Tn10
Parent Donor AQ9239
Parent Recipient AQ666
Methods TD./PI
Latest Marker Spc-r, H2O2-s, ΔpolB::spc
Selection Tc-r
Source
Gene Mutants Detail Info
Reference Kogoma T, Maldonado RR.
DNA polymerase I in constitutive stable DNA replication in Escherichia coli.
J Bacteriol  (1997)  179(7)   2109-15  
[PubMed ID = 9079893]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.
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