Gene Mutants - Detail

ME No. ME9590
Strain Name AQ10452
Sex F-
Plasmid Integrated
Plasmid Autonomous
Genotype thr-1 leu-6 thi-1 lacY1 galK2 ara-14 xyl-5 mtl-1 proA2 his-4 argE3 rpsL-31 tsx-33 sup-37 thyA sfiA11 pyrD::Tn5
Parent Donor AQ1023
Parent Recipient AQ8846
Methods TD./PI
Latest Marker less filamentous after UV irradiation, sfiA11
Selection Km-r
Reference Kogoma T, Cadwell GW, Barnard KG, Asai T.
The DNA replication priming protein, PriA, is required for homologous recombination and double-strand break repair.
J Bacteriol  (1996)  178(5)   1258-64  
[PubMed ID = 8631700]  [RRC reference
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), Uracil (50 μg/ml), kanamycin (55μg/ml).
Other Remarks Further information: Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.