Gene Mutants - Detail

ME No. ME9588
Strain Name AQ10303
Sex F-
Plasmid Integrated
Plasmid Autonomous
Genotype ara Δ(lac-pro) fis::kan rpsL thi rnhA224
Parent Donor AQ798
Parent Recipient AQ7996
Methods TD./PI
Latest Marker Ls, rnh224
Selection Tc-r, Km-r
Reference Bates DB, Asai T, Cao Y, Chambers MW, Cadwell GW, Boye E, Kogoma T.
The DnaA box R4 in the minimal oriC is dispensable for initiation of Escherichia coli chromosome replication.
Nucleic Acids Res  (1995)  23(16)   3119-25  
[PubMed ID = 7667087]  [RRC reference
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml).
Other Remarks Ls: Srm phenotype, sensitivity for rich media. Ref: Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.