Gene Mutants - Detail

ME No. ME9575
Strain Name AQ10033
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype trpA9605 his-29 pro ilv metB1 thyA deoB (or deoC) rnhA224 gidA95::kan
Parent Donor AQ7664
Parent Recipient AQ666
Methods TD./PI
Latest Marker gidA95::kan
Selection Km-r
Source
Gene Mutants Detail Info
Reference Bates DB, Asai T, Cao Y, Chambers MW, Cadwell GW, Boye E, Kogoma T.
The DnaA box R4 in the minimal oriC is dispensable for initiation of Escherichia coli chromosome replication.
Nucleic Acids Res  (1995)  23(16)   3119-25  
[PubMed ID = 7667087]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.
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