Gene Mutants - Detail

ME No. ME9574
Strain Name AQ9999
Sex F-
Plasmid Integrated
Plasmid Autonomous
Genotype trpA9605 his-29 pro ilv metB1 thyA deoB(or deoC) lrp-201::Tn10
Parent Donor AQ9950
Parent Recipient AQ634
Methods TD./PI
Latest Marker lrp-201
Selection Tc-r
Reference Hong X, Cadwell GW, Kogoma T.
Activation of stable DNA replication in rapidly growing Escherichia coli at the time of entry to stationary phase.
Mol. Microbiol.  (1996)  21(5)   953-61  
[PubMed ID = 8885266]  [RRC reference
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks