Gene Mutants - Detail

ME No. ME9574
Strain Name AQ9999
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype trpA9605 his-29 pro ilv metB1 thyA deoB(or deoC) lrp-201::Tn10
Parent Donor AQ9950
Parent Recipient AQ634
Methods TD./PI
Latest Marker lrp-201
Selection Tc-r
Source
Reference Hong X, Cadwell GW, Kogoma T.
Activation of stable DNA replication in rapidly growing Escherichia coli at the time of entry to stationary phase.
Mol. Microbiol.  (1996)  21(5)   953-61  
[PubMed ID = 8885266]  [RRC reference
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks
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