Gene Mutants - Detail

ME No. ME9573
Strain Name AQ9964
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype trpA9605 his-29 pro ilv metB1 thyA deoB(or C) recG258::miniTn10kan Δ(srlR-recA)306 srlC300::Tn10
Parent Donor AQ663
Parent Recipient AQ8130
Methods TD./PI
Latest Marker UVs, ΔrecA306
Selection Tc-r
Source
Gene Mutants Detail Info
Reference Hong X, Cadwell GW, Kogoma T.
Escherichia coli RecG and RecA proteins in R-loop formation.
EMBO J  (1995)  14(10)   2385-92  
[PubMed ID = 7774596]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
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