Gene Mutants - Detail

ME No. ME9569
Strain Name AQ9897
Sex F-
Plasmid Integrated
Plasmid Autonomous Other
Genotype trpA9605 his-29 pro ilv metB1 thyA deoB (or deoC) recG258::miniTn10kan rnhA339::cat
Parent Donor
Parent Recipient AQ9853
Methods TF.
Latest Marker
Selection Km-r
Reference Hong X, Cadwell GW, Kogoma T.
Escherichia coli RecG and RecA proteins in R-loop formation.
EMBO J.  (1995)  14(10)   2385-92  
[PubMed ID = 7774596]  [RRC reference
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), kanamycin (55μg/ml).
Other Remarks Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.