Gene Mutants - Detail

ME No. ME9563
Strain Name AQ9833
Sex F-
Plasmid Integrated
Plasmid Autonomous Other
Prophage λ+ λ[sfiA::lacZ cI(ind-)]
Genotype thr-1 leu-6 thi-1 lacY1 galK2 ara-14 xyl-5 mtl-1 proA2 his-4 argE3 rpsL-31 tsx-33 sup-37 thyA deoB (orC) lacZU118 lacI42::Tn10 λp[sfiA::lacZ cI(Ind-)]
Parent Donor
Parent Recipient AQ9789
Methods TF.
Latest Marker pHKχ1-cos, pFM123
Selection Km-r, Ap-r
Reference Asai T, Bates DB, Kogoma T.
DNA replication triggered by double-stranded breaks in E. coli: dependence on homologous recombination functions.
Cell  (1994)  78(6)   1051-61  
[PubMed ID = 7923355]  [RRC reference
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), ampicillin (40μg/ml), kanamycin (55μg/ml).
Other Remarks cultured at 30C
Further information: Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.