Gene Mutants - Detail

ME No. ME9556
Strain Name AQ9717
Sex F-
Plasmid Integrated
Plasmid Autonomous
Genotype recN1502::Tn5 his-4 argE3 leuB6 proA2 thr-1 thi-1 rpsL31 galK2 lacY1 ara-14 xyl-5 mtl-1 kdg-51 supE44 tsx-33 thyA zga::Tn10 deoB (orC)
Parent Donor AQ5786
Parent Recipient AQ8224
Methods TD./PI
Latest Marker low thymide, thyA
Selection Tc-r
Reference Asai T, Bates DB, Kogoma T.
DNA replication triggered by double-stranded breaks in E. coli: dependence on homologous recombination functions.
Cell  (1994)  78(6)   1051-61  
[PubMed ID = 7923355]  [RRC reference
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.