Gene Mutants - Detail

ME No. ME9550
Strain Name AQ9643
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage λ+ λDB101(gidA+)
Genotype trpA9605 his-29 pro ilv metB1 thyA deoB (or deoC) asnA101::cat
Parent Donor λDB101
Parent Recipient AQ7664
Methods OTHER METHODSλ infection
Latest Marker Km-s, gidA+
Selection Cm-r
Source
Gene Mutants Detail Info
Reference Bates DB, Asai T, Cao Y, Chambers MW, Cadwell GW, Boye E, Kogoma T.
The DnaA box R4 in the minimal oriC is dispensable for initiation of Escherichia coli chromosome replication.
Nucleic Acids Res  (1995)  23(16)   3119-25  
[PubMed ID = 7667087]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Asn (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks
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