Gene Mutants - Detail

ME No. ME9549
Strain Name AQ9601
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype thr-1 leu-6 thi-1 lacY1 galK2 ara-14 xyl-5 mtl-1 proA2 his-4 argE3 rpsL-31 tsx-33 sup-37 thyA recD1903::miniTc
Parent Donor AQ8070
Parent Recipient AQ5004
Methods TD./PI
Latest Marker recD1903
Selection Tc-r
Source
Reference Asai T, Bates DB, Kogoma T.
DNA replication triggered by double-stranded breaks in E. coli: dependence on homologous recombination functions.
Cell  (1994)  78(6)   1051-61  
[PubMed ID = 7923355]  [RRC reference
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks
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