Gene Mutants - Detail

ME No. ME9548
Strain Name AQ9561
Sex F-
Plasmid Integrated
Plasmid Autonomous
Genotype thr-1 leuB6 thi-1 lacY1 galK2 ara-4 xyl-5 mtl-1 proA2 his-60 argE3 rpsL31 tsx-33 supE44 recB21 recC22 sbcA23 Δ(srlR-recA)306 srlC300::Tn10
Parent Donor AQ7982
Parent Recipient AQ3625
Methods TD./PI
Latest Marker UVs, ΔrecA306
Selection Tc-r
Reference Asai T, Bates DB, Kogoma T.
DNA replication triggered by double-stranded breaks in E. coli: dependence on homologous recombination functions.
Cell  (1994)  78(6)   1051-61  
[PubMed ID = 7923355]  [RRC reference
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.