Gene Mutants - Detail

ME No. ME9545
Strain Name AQ9513
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage λ+ λGM108A (rnhA+ dnaAQ::lacZ)
Genotype metE90 trpA9605 lacI22 lacZ118 rpsL171 azi-9 nalA18 thyA708 deo-29 rnhA102 recA200 sfiA11 polA25::spc zih-35::Tn10λGM108A (rnhA+ dnaAQ::lacZ)
Parent Donor λGM108A
Parent Recipient AQ8633
Methods OTHER METHODSλ infection
Latest Marker λGM108A (rnhA+ dnaAQ::lacZ)
Selection Blue colony on X-gal plate
Source
Gene Mutants Detail Info
Reference Cao Y, Kogoma T.
The mechanism of recA polA lethality: suppression by RecA-independent recombination repair activated by the lexA(Def) mutation in Escherichia coli.
Genetics  (1995)  139(4)   1483-94  
[PubMed ID = 7789754]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Asn (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
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