Gene Mutants - Detail

ME No. ME9543
Strain Name AQ9507
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype lacZ53 rpsL151 thyA36 polA12 rha-5 deoC2 IN(rrnD-rrnE)1 Δ(srlR-recA)306
Parent Donor AQ663
Parent Recipient AQ6039
Methods TD./PI
Latest Marker UVs, ΔrecA306
Selection Tc-r
Source
Gene Mutants Detail Info
Reference Cao Y, Kogoma T.
The mechanism of recA polA lethality: suppression by RecA-independent recombination repair activated by the lexA(Def) mutation in Escherichia coli.
Genetics  (1995)  139(4)   1483-94  
[PubMed ID = 7789754]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml).
Other Remarks Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
Request
page-top
/ecoli/strain