Gene Mutants - Detail

ME No. ME9542
Strain Name AQ9504
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage λ+ λp[sfiA::lacZ cI(Ind-)]
Genotype trpA9605 his-29 pro ilv metB1 thyA deoB (or deoC) Δ(lacIPOZY)x74 proC::Tn5 λp[sfiA::lacZ cI(Ind-)]
Parent Donor λ[sfiA::lacZ]
Parent Recipient AQ8820
Methods TD./Others PENICILLIN OTHER METHODSλ infection
Latest Marker λp[sfiA::lacZ cI(Ind-)]
Selection Lac+
Source
Gene Mutants Detail Info
Reference Hong X, Cadwell GW, Kogoma T.
Activation of stable DNA replication in rapidly growing Escherichia coli at the time of entry to stationary phase.
Mol Microbiol  (1996)  21(5)   953-61  
[PubMed ID = 8885266]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Asn (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks Further information: Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
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