Gene Mutants - Detail

ME No. ME9517
Strain Name AQ9179
Sex F-
Plasmid Integrated
Plasmid Autonomous Other
Prophage
Genotype trpA9605 his-29 pro ilv metB1 thyA deoB (or deoC) rnhA224 Δ(srlR-recA)306
Parent Donor
Parent Recipient AQ8969
Methods TF.
Latest Marker pAQ7985 (miniF::recA730)
Selection Km-r
Source
Gene Mutants Detail Info
Reference Kogoma T, Barnard KG, Hong X.
RecA, Tus protein and constitutive stable DNA replication in Escherichia coli rnhA mutants.
Mol Gen Genet  (1994)  244(5)   557-62  
[PubMed ID = 8078483]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), kanamycin (55μg/ml).
Other Remarks Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.
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