Gene Mutants - Detail

ME No. ME9510
Strain Name AQ9077
Sex F-
Plasmid Integrated
Plasmid Autonomous
Genotype metE46 trp-3 his-4 thi-1 galK2 lacY1 (or lacZ4) mtl-1 ara-9 tsx-3 ton-1 rpsL8 (or rpsL9) supE44 thyA zga::dTn10(Tc-s) rnhA339::cat recB270(Ts) oriCdel-1071 zif90::pML31
Parent Donor AQ3634
Parent Recipient AQ8964
Methods TD./PI
Latest Marker Ts, recB270
Selection Thy+
Reference Hong X, Kogoma T.
Absence of a direct role for RNase HI in initiation of DNA replication at the oriC site on the Escherichia coli chromosome.
J Bacteriol  (1993)  175(20)   6731-4  
[PubMed ID = 8407851]  [RRC reference
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), chloramphenicol (25μg/ml).
Other Remarks Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.