Gene Mutants - Detail

ME No. ME9506
Strain Name AQ9016
Sex F-
Plasmid Integrated
Plasmid Autonomous F'
Prophage
Genotype hsdR514(r- m+) supE44 supF58 lacY1 or Δ(lacIZY)6 galK2 galT22 metB1 trpR55 ΔpolA::kan zih-35::Tn10
Parent Donor AQ6466
Parent Recipient AQ8866
Methods TD./PI
Latest Marker UVs, ΔpolA::kan
Selection Tc-r
Source
Gene Mutants Detail Info
Reference Cao Y, Kogoma T.
Requirement for the polymerization and 5'-->3' exonuclease activities of DNA polymerase I in initiation of DNA replication at oriK sites in the absence of RecA in Escherichia coli rnhA mutants.
J Bacteriol  (1993)  175(22)   7254-9  
[PubMed ID = 8226672]  [RRC reference] 
Culture Condition Recommended medium:
Luria broth supplemented with glucose (0.1%).
Other Remarks Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.
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